J-Express microarray analysis course 22nd-24th november
Here you will find important information regarding the prerequisites for the course, and also the files needed for the course. In addition you will find the exercises and lecture handouts here.
Installing J-Express on your laptop
In this course you will need to bring your own laptop with the J-Express software installed to use for the exercises during the course. Instructions on how to install J-Express and test that it's running properly can be found here.
Program
Here is the program for the course. Note that timepoints are tentative.
Miscellaneous
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Transportation
The Research building/Forskningsbygget is situated next to the Radium Hospital at Montebello in Oslo, and is easy accessed by public transporttion with Metro line 6 Jar/Åsjordet to the stop "Montebello", and buses 23 Lysaker, 28 Fornebu and 42 Kolsås to the "Montebelloveien" stop. Map with essential places marked can be found here.
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Lunch
Bring your own lunch, or buy it in the cafeteria at Forskningsbygget. NB: The cafeteria only accepts payment by card (VISA, BankAxept) and no cash.
Files needed for the course
- RatBrainProfiling.pro
- RatBrainCortex.pro
- GeneSets:MSigDB
- gene_ontology.obo.zip
- gene_association.rgd.zip
- c2.all.v2.5.symbols.gmt
Print outs for lectures (print yourself if you want one)
Lecture 1: Technology introduction
Lecture2: Microarray pipeline and pre-processing
Lecture 3: Introduction of course dataset
Lecture 4: Basics and fundamentals of J-Express
Lecture 5: Experimental design
Lecture 6: Explorative approaches
Lecture 7: Differential expression
Lecture 8: Gene ontology overrepresentation analysis (GO)
Lecture 9: Gene ser enrichment analysis (GSEA)
Lecture 10: From raw data to publication (will be handed out at the course)
Hand outs for expercises (will be provided at the course)
Exercise 1: Basics
Exercise 2: Fundamentals
Exrecise 3: Explorative approaches
Exercise 4: Differential expression
Exercise 5: Gene ontology overrepresentation analysis
Exercise 6: Gene set enrichment analysis